restriction fragment length polymorphism con

doi:10.1007/s0028-5.Terminal restriction fragment length polymorphism (T-RFLP) analysis is a widespread technique for rapidly fingerprinting microbial communities. Development of a simple and rapid method based on polymerase chain reaction-based restriction fragment length polymorphism analysis to differentiate Helicobacter, Campylobacter, and Arcobacter species. González A, Moreno Y, González R, Hernández J, Ferrús MA. Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food. Meyer R, Höfelein C, Lüthy J, Candrian U. Advances in the use of terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes to characterize microbial communities. Schütte UME, Abdo Z, Bent SJ, Shyu C, Williams CJ, Pierson JD, et al. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for rapid diagnosis of neonatal sepsis. Rohit A, Maiti B, Shenoy S, Karunasagar I. Cleaved Amplified Polymorphic Sequences (CAPS) n.d. Restriction Fragment Length Polymorphism (RFLP) n.d. This study provides importance of such technique in PJI setting where it is very important to identify pathogens as it has huge implications in the management. Rohit et al., used this technique to rapidly diagnose bacterial species in the setting of neonatal sepsis. Using PCR RFLP method with 16s bacterial DNA has been used in bacterial identification in clinical situations, food safety and also identify different strains of bacteria. Combining PCR along with RFLP (also called cleaved amplified polymorphic sequences or CAPS) solves the problem of the requirement of a large sample. This process requires a large amount of DNA and is labor intensive. RFLP probes are widely used in genome mapping and variation analysis such as genotyping, forensics, paternity tests, hereditary disease diagnostics, etc. Restriction fragment length polymorphism (RFLP) is a difference in homologous DNA sequences which are identified by the different length of sequences after digestion of DNA samples using specific restriction endonucleases. One advantage of this technique is the amount of time required to get the pathogen identification is approximately 3 to 4 hours compared to multiple days for microbiological culture methods. Researchers isolated a broad range of bacteria including fastidious organisms like Chlamydophila pneumonia, Stenotrophomonas maltophilia, Brucella melitensis. This was superior compared to the culture where sensitivity and specificity was 31.6% and 100%. Results showed that using PCR RFLP Sensitivity and specificity was found to be 97.4% and 100% respectively. 50% of the samples were deemed infected based on the above criteria. International consensus meeting criteria were used to identify prosthetic joint infection. The study assessed 76 samples using this technique. Researchers also obtained bacterial cultures at the same time.

The main aim of this prospective study was to assess the diagnostic accuracy of polymerase chain reaction (PCR) using RFLP (restriction fragment length polymorphism) method. Moshirabadi A, Razi M, Arasteh P, Sarzaeem MM, Ghaffari S, Aminiafshar S, Hosseinian Khosroshahy K, Sheikholeslami FM. Paper of the week: Polymerase chain reaction assay using the restriction fragment length polymorphism technique in the detection of prosthetic joint infections: A Multi-Centered Study.

Restriction fragment length polymorphism con